Table 1. Summary of groups identified following hierarchical clustering of expression data.
| Groupa | Expression profileb | Number of genes | GO Termsc | ||||||
| 0 | 1 | 3 | 7 | 17 | 29 | 31 | |||
| GO:30414: protease inhibitor activity | |||||||||
| GO:4866: endopeptidase inhibitor activity | |||||||||
| I | - | - | - | - | ↑ | ↑ | - | 396 | GO:4857: enzyme inhibitor activity |
| GO:30234: enzyme regulator activity | |||||||||
| GO:4867: serine-type endopeptidase inhibitor activity | |||||||||
| GO:3676: nucleic acid binding | |||||||||
| GO:3735: structural constituent of ribosome | |||||||||
| II | - | - | - | - | ↓ | ↑ | - | 255 | GO:5198: structural molecule activity |
| GO:3723: RNA binding | |||||||||
| GO:8026: ATP-dependent helicase activity | |||||||||
| GO:3824: catalytic activity | |||||||||
| GO:16787: hydrolase activity | |||||||||
| III | ↓ | - | - | - | - | - | - | 301 | GO:45156: electron transporter, in the cyclic electron transport pathway of photosynthesis |
| GO:30554: adenyl nucleotide binding | |||||||||
| GO:17076: purine nucleotide binding | |||||||||
| GO:46857: oxidoreductase activity, with NAD or NADP as acceptor | |||||||||
| GO:16661: oxidoreductase activity | |||||||||
| IV | - | - | - | - | - | - | - | 158 | GO:20037: heme binding |
| GO:46906: tetrapyrrole binding | |||||||||
| GO:5506: iron ion binding | |||||||||
| GO:50589: leucocyanidin oxygenase activity | |||||||||
| V | - | - | - | - | ↓ | ↓ | - | 211 | GO:16210: naringenin-chalcone synthase activity |
| GO:4097: catechol oxidase activity | |||||||||
| GO:5198: structural molecule activity | |||||||||
| GO:4871: signal transducer activity | |||||||||
| GO:4672: protein kinase activity | |||||||||
| VI | - | - | ↑ | ↑ | - | - | - | 223 | GO:4872: receptor activity |
| GO:3700: transcription factor activity | |||||||||
| GO:4674: protein serine/threonine kinase activity | |||||||||
| GO:8061: chitin binding | |||||||||
| GO:1871: pattern binding | |||||||||
| VII | - | - | - | ↑ | ↑ | - | - | 85 | GO:30247: polysaccharide binding |
| GO:4568: chitinase activity | |||||||||
| GO:30246: carbohydrate binding | |||||||||
| GO:4289: subtilase activity | |||||||||
| VIII | - | - | - | ↑ | - | - | - | 30 | GO:42802: protein self binding |
| GO:4252: serine-type endopeptidase activity | |||||||||
distinct patterns of gene expression were identified following hierarchical clustering [42] of the significantly differentially expressed genes (Figure S1).
Average expression profiles were calculated in GeneSpring for each group of genes across the time series. Column headers represent time (d) relative to Pi withdrawal, with Pi being re-supplied after 28 d. Symbols: ↑ average group gene expression up-regulated more than 1.5-fold, ↓ average group gene expression down-regulated more than 1.5-fold, - average group gene expression between 1.5 down-regulated and 1.5-fold up-regulated, i.e. no change.
Top five Gene Ontology terms present in each group that appear more often than would be expected by chance, identified using GeneSpring's GO Ontology Browser.