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. 2011 Sep 14;6(9):e24768. doi: 10.1371/journal.pone.0024768

Figure 2. TCF-1-/- T cells have a high potential to form Th17 cells.

Figure 2

(A) Isolated naïve CD4+ T cells from WT control or TCF-1-/- mice were differentiated in neutral conditions (without cytokines) or Th17 conditions (TGFβ + IL-6) for three days. Intracellular IL-17A was then stained and analyzed by flow cytometry. (B) Percentage of IL-17A producing cells as averaged (mean±SEM) from six independent experiments performed as described in A. (C) mRNA levels of IL-17A, IL-17F and IL-23R in ThN and Th17 cells described in A were determined by quantitative RT-PCR; mean±SEM from four independent experiments performed in triplicate.