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. 2011 Sep 14;6(9):e24879. doi: 10.1371/journal.pone.0024879

Figure 1. Neuronatin is expressed in the P21 rat hippocampus.

Figure 1

(A) Nnat in situ hybridization (ISH) in hippocampus visualized using alkaline phosphatase. Nnat mRNA is present in hippocampal regions CA2 and 3. Arrowheads denote dendritic localization. Scale bar: 20 µm. Schematic of cell body and dendritic layer boundary in 40× image is shown (bottom right panel). (B) NNAT immunofluorescent staining in the CA2 region of hippocampus showing dendritic localization, NNAT (red), MAP2 (green). Scale bar: 20 µm. (C) left, Western blot for NNAT and right, RT-PCR for Nnat mRNA using rat hippocampal tissue showing both α and β isoforms.