Table 3.
Interference of high and low chlorine on real-time PCR detection of P. aeruginosa. For comparison, another set of samples were neutralized before real-time PCR. SRA (somatic cell releasing agent) was used to remove possible non-bacterial cells present in pool samples and to adjust pH. CT values are shown and converted CFU/PCR values are provided in parenthesis. Tests were done in duplicate.
| Swimming pool water (12 mg·L−1 of total chlorine) | Neutralized swimming pool water (0 mg·L−1 of total chlorine) | |||
|---|---|---|---|---|
| P. aeruginosa strain used for spiking | No SRA treatment | SRA treatment | No SRA treatment | SRA treatment |
| MSH10 | 31.67 ± 0.26 (1.6 ± 0.2 × 103) | 32.72 ± 0.00 (0.9 ± 0.0 × 103) | 18.24 ± 0.01 (1.8 ± 0.0 × 106) | 17.71 ± 0.08 (2.4 ± 0.1 × 106) |
| MSH3 | 32.47 ± 0.23 (1.1 ± 0.1 × 103) | 30.24 ± 0.01 (3.4 ± 0.0 × 103) | 22.69 ± 0.10 (1.8 ± 0.1 × 105) | 22.82 ± 0.03 (1.7 ± 0.0 × 105) |
| Tap water (0.7 mg·L−1 of total chlorine) | Neutralized tap water (0 mg·L−1 of total chlorine) | |||
| PAO1 | 25.34 ± 0.12 (4.4 ± 0.3 × 104) | 26.60 ± 0.06 (2.3 ± 0.1 × 104) | 25.99 ± 0.08 (3.2 ± 0.1 × 104) | 26.17 ± 0.07 (2.9 ± 0.1 × 104) |