Effects of Y27632 and nicardipine on basal pT696, and pT853 in antrum, proximal colon, and fundus smooth muscles. Top panels, representative western blots, 20 μg protein/lane. Middle panels, pT696, cumulative data of average density values (+/− SD). Bottom panels, pT853, cumulative data of average density values (+/− SD). C, control; nic, nicardipine (1μM). * average density values significantly different from control values (P<0.05). At least 4 antrum, fundus, and proximal colon smooth muscle homogenates from each treatment were analyzed by densitometry of duplicate or triplicate western blots.