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. 2003 Nov 25;2:40. doi: 10.1186/1476-4598-2-40

Figure 2.

Figure 2

RT-PCR analysis for CUL-1, -2, -3, -4A, -4B, -5, and ribosomal protein S9 in HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells. RT-PCR was performed using 1 μg of total RNA and 25 cycles of PCR. For all of the cells examined, a 560-bp cDNA for CUL-1, a 358-bp cDNA for CUL-2, a 613-bp cDNA for CUL-3, a 512-bp cDNA for CUL-4A, a 593-bp cDNA for CUL-4B, a 674-bp cDNA for CUL-5, and a 411-bp cDNA for ribosomal protein S9 were amplified by RT-PCR. The results shown are representative of duplicate experiments using independent RNA samples.