Table 1.
Primers used for RT-PCR analysis of CUL family members
| Primer Sequences | Primer Location and GenBank Accession # |
Annealing Temp. and Predicted product size |
Restriction endonuclease and cut site |
|
CUL-1 forward: 5'-CGCTGGCTTTGTGGCTGCTC-3' CUL-1 reverse: 5'-TGTGGCGGCTGGCGTAGAA-3' |
nt 1510 – 1529 nt 2051 – 2069 XM027270 |
58°C 560-bp |
BglII 1891 |
|
CUL-2 forward: 5'-CAGCGAAAGGGATGACAGAGAATG-3' CUL-2 reverse: 5'-TGAGTAAGAGGCCACGCACCA-3' |
nt 1351 – 1374 nt 1688 – 1708 XM005814.3 |
54°C 358-bp |
AatII 1435 |
|
CUL-3 forward: 5'-AGAGCGGAAAGGAGAAGTCGTAGA-3' CUL-3 reverse: 5'-CTCAAAGTCACCCGCAATAGTT-3' |
nt 833 – 856 nt 1424 – 1445 XM051985.1 |
53°C 613-bp |
EcoRI 1160 |
|
CUL-4A forward: 5'-AACTCCACGCTGCCCTCCATCTG-3' CUL-4A reverse: 5'-TGCTGCCCGCCCCTCACC-3' |
nt 346 – 368 nt 840 – 857 XM027482.1 |
58°C 512-bp |
Sau3AI 452 |
|
CUL-4B forward: 5'-GCGGAGCTGCTTTCACCA-3' CUL-4B reverse: 5'-TTCTTCAACCGTTTCTTTCATCTG-3' |
nt 1400 – 1417 nt 1969 – 1992 NM003588.1 |
54°C 593-bp |
HaeIII 1551 |
|
CUL-5 forward: 5'-GAGTGGCTAAGAGAAGTTGGTATG-3' CUL-5 reverse: 5'-TCTTCTCTCATCCTTTCTGTAGTG-3' |
nt 1521 – 1544 nt 2171 – 2194 NM003478.1 |
58°C 674-bp |
EcoRI 1767 |
|
Ribosomal S9 forward: 5'-GCAGGCGCAGACGGGGAAGC-3' Ribosomal S9 reverse: 5'-CGCGAGCGTGGTGGATGGAC-3' |
nt 6 – 25 nt 397 – 416 NM001013.2 |
62°C 411-bp |
DdeI 349 |