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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Aging Cell. 2011 Jun 14;10(5):807–823. doi: 10.1111/j.1474-9726.2011.00721.x

Figure 9. MPP+-induced mitochondrial fragmentation preceded and was prerequisite for cell death.

Figure 9

SH-SY5Y cells seeded on 96 well plates were treated with 2.5 mM MPP+ and cell death/viability was determined by LDH release and MTT assays after various periods of incubation time (A). (*p<0.05, when compared with the control cells without MPP+ treatment; student-t-test.) (B) Representative immunoblot pictures of cleaved caspase 3 and full length caspase 3 in SH-SY5Y cells treated with 2.5 mM MPP+at different time points. Equal protein amounts (10 μg) were loaded and actin was used as an internal loading control. (C) Measurement of cell death by LDH assay in control, negative control and DLP1 RNAi SH-SY5Y cells treated with 2.5 mM MPP+ at different time points. (*p<0.05, when compared with the control cells with MPP+ treatment; student-t-test). All experiments were repeated three times.