Table 4.

Genes showing disruption to the balance of alternatively-spliced isoforms by real-time PCR.

Gene	id	isoform	Mean	se	Comparison	Coeff	CI	CI	p value
BCL11B
1	hs01102259_m1	NM_138576.2	1.058325	0.349844
2	hs01112109_m1	NM_022898.1	1.062152	0.36903	1 over 2	0.00125	−0.0000175	0.0025182	0.053
EFNA1
3	hs00358887_m1	NM_004428.2	1.376142	1.084887
4	hs01020895_m1	NM_182685.1	1.315693	1.144735	3 over 4	−0.00394	−0.0078545	−0.0000253	0.049
GPR18
5	hs00245542_m1	NM_001098200.1	1.070176	0.407375
6	hs01649814_m1	NM_005292.3	1.081336	0.446335	5 over 6	0.00189	3.60E-06	0.0037757	0.05
VCAN
7	hs01007937_m1	NM_001164097.1	1.098376	0.559999
8	hs01007941_m1	NM_001126336.2	1.086235	0.457961	7 over 8	0.002647	0.0001322	0.0051619	0.039
9	hs01007943_m1	NM_001164098.1	1.23125	0.845863	9 over 7	−0.01048	−0.0193924	−0.0015691	0.022
10	hs01007944_m1	NM_004385.4	1.210006	0.92149	9 over 10	−0.00778	−0.0154129	−0.0001444	0.046
ANXA7
11	hs00244504_m1	NM_004034.2	1.066305	0.388382	12 over 11
12	hs00559416_m1	NM_001156.3	1.929157	8.420153	11 over 12	−0.00219	−0.0057466	0.0013578	0.223
CXCR5
13	hs00540527_s1	NM_001716.3	1.116763	0.540786	14 over 13
14	hs00540548_s1	NM_032966.1	1.100177	0.497535	13 over 14	−0.0011	−0.0026285	0.0004256	0.156
IGL6ST
15	hs01006741_m1	NM_002184.3	1.068342	0.397245	16 over 15
16	hs01006742_m1	NM_175767.2	1.898088	5.478007	15 over 16	0.004691	−0.0045713	0.0139524	0.317
PUM1
17	hs00982771_m1	NM_001020658.1	1.031583	0.253846
18	hs00985111_m1	NM_014676.2	1.091318	0.995023	17 over 18	0.001596	−0.0003754	0.0035682	0.111

The ratio of alternatively expressed isoforms of specific genes as determined by real-time PCR is correlated with age in the table above. Association with age is calculated by logistic regression. Id = probe identity, isoform = transcript identity, Mean = mean ratio, se = standard error, comparison indicates which comparisons were made for the analysis, coeff = correlation coefficient, CI = confidence intervals. The analysis was carried out on 100 individuals.