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. Author manuscript; available in PMC: 2012 Sep 14.
Published in final edited form as: Nano Lett. 2011 Aug 1;11(9):3720–3726. doi: 10.1021/nl201978c

Figure 7. Cellular targeting of human folate receptor with DSPE-PEG coated iron oxide nanoparticles.

Figure 7

Human folate receptors on two cancer cell lines, MDA-MB-435s and HeLa, were detected with 17 nm IONPs. The IONPs were conjugated with goat anti-human folate receptor-1 antibody and labeled with a fluorescence molecule, DiI (ex/em = 549/580 nm). A and B are fluorescence images of MDA-MB-435s and HeLa cells, respectively. Cell nuclei were counterstained with Hoechst 33342. C. Western blots of human folate receptor (arrow). Lanes 1 and 2 are MDA-MB-435s and HeLa cells respectively. D. Flowcytometry analysis of the two cell lines treated with IONPs conjugated with the anti-folate receptor antibody or a non-targeting control IgG. All IONPs were labeled with DiI.