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Monolayer Penetration Experiments of GRP1-PH and Respective Mutations
Insertion of the wild-type GRP1 PH domain (filled circles), V278E (filled triangles), Y298E (filled squares), A346E (filled diamonds), and V351E (open squares) mutations into a POPC/POPE/PI(3,4,5)P3 (77:20:3) monolayer in a subphase of 10 mM HEPES/0.16 M KCl (pH 7.4) monitored as a function of π.
