Fig. 1.

Promastigotes and Δcpb amastigotes mediate increased MAP kinase signalling in macrophages. Cells (1 × 10⁶/well) were infected with L.mexicana promastigotes (A–C) or Δcpb amastigotes (D-F) (ratio, 5:1) for the times indicated. Whole cell lysates were prepared, separated by SDS-PAGE, and then assessed for p-JNK1/2 and T-JNK (A and D), p-ERK1/2 and T-ERK (B and E) p-p38 MAPK and T-p38MAPK (C and F) as outlined in Section 2. The results are representative of 3 independent experiments with similar findings.