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Schematic principle of microdialysate sampling and two dimensional electrophoresis. Following the parietal implantation of the microdialysis catheter, a small volume of microdialysate is collected. The microdialysate sample is subjected two-dimensional gel electrophoresis with first dimension IEF and second dimension SDS-PAGE. Gels were stained and individual protein spots were analysed by mass spectrometry.
