Table 5.
Representative animal models of excisional wound infections
| Animal species | Microorganism & inoculum | Methods used to produce infections | Wound area | End point | Refs |
| Male BALB/c mouse | E. coli 5 × 106 CFU | Four full-thickness excisional wounds were made in a line along the dorsal surface. Wounds measured 8 × 12.5 mm2. The bottom of the wound was panniculus carnosus. A bacterial suspension (50 µL) was inoculated into each wound using a yellow tip. |
8 × 12.5 mm | Infected wounds in living mice showed only a slight loss of bacterial luminescence over a period of 4 h. The next day, control infected wounds in living mice had lost, on an average, 90% of the original luminescence signal. | 100 |
| Female mutant diabetic mouse | S. aureus 1 × 105 CFU | Two round, full-thickness wounds were prepared on the back of each mouse using a punch biopsy instrument (3 mm diameter). Each wound was covered with a sterilized transparent dressing and inoculated with 105 CFU MRSA or MSSA in 30 µL of PBS. |
3 mm diameter | Wounding and infection caused by MRSA on the back of the diabetic mice significantly induced increased CFU on wounds compared to those of the MRSA-infected normal mice. |
105 |
| Male Wistar rat | Not infected | A full thickness of the excision wound of circular area 400 mm2 and 2 mm depth was created. | 4 cm2 | 108 | |
| Female domestic pig | Not infected | Excisional dermal wounds (n = 10; two sets of 5) were created on the back of each pig. Full-thickness sections of skin (1 × 1 in.) were removed during the wounding process. | 1 × 1 in n = 10 | 113 | |
| Female Yorkshire pig (diabetic and non-diabetic) | S. aureus 2 × 108 CFU | Fourteen full thickness excisional wounds (1.5 × 1.5 × 0.5 cm) were created using a blade. 1 mL of the bacterial suspension was injected into each chamber. |
1.5 × 1.5 cm | Diabetic wounds showed a sustained significant infection compared to non-diabetic wounds over the whole time course of the experiment. | 114 |
| New Zealand White rabbit with both sexes | Not infected | Two matching, full-thickness, square, cutaneous wounds approximately 2 cm × 2 cm were then created on the right and left dorsal hindquarters. | 2 × 2 cm | 116 |