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. 2011 Sep;134(1):41–49. doi: 10.1111/j.1365-2567.2011.03464.x

Figure 3.

Figure 3

CD45RO+ natural killer (NK) cells from pleural fluid cells (PFCs) produced more interferon-γ (IFN-γ) compared with CD45RO− NK cells under stimulation with interleukin-12 (IL-12). (a) Freshly purified NK cells from peripheral blood mononuclear cells (PBMCs) labelled with CFSE were co-incubated with purified NK cells from PFCs. Cells were stimulated with or without PMA and ionomycin in the presence of brefeldin A for 4 hr. The expression of IFN-γ was analysed by FACS. Representative dot plots of three independent experiments were shown. (b) Purified NK cells from PFCs or PBMCs were incubated with or without PMA and ionomycin. The expression of IFN-γ in supernatants was detected by ELISA. Results were given as mean ± SD. *P < 0·05 was considered significant. (c) Freshly sorted CD56+ CD16− NK cells, CD56+ CD16+ NK cells and total NK cells from PFCs were stimulated with or without PMA and ionomycin. The expression of IFN-γ in supernatants was evaluated by ELISA. (d) PFCs were stimulated with or without IL-12 or PMA plus ionomycin for 4 hr in the presence of brefeldin A. The expression of IFN-γ was evaluated by FACS. Representative dot plots of three independent experiments were shown with similar results. (e) Statistical results of IFN-γ expression by CD45RO+ or CD45RO− NK cells from PFCs were shown as mean ± SD in histogram. *P < 0·05 was considered significant.