Table 2.
Effect of Wortmannin (WM) on secretion of cytokines from mature monocyte-derived dendritic cells
| Cytokine | 0 μm WM (pg/ml ± SD) | 1 μm WM (pg/ml ± SD) | P value |
|---|---|---|---|
| Interleukin-6 | 10608 ± 6870 | 25650 ± 8160 | < 0·01 |
| Interleukin-12 | 23426 ± 17328 | 49950 ± 23017 | < 0·1 |
| Interleukin-23 | 533 ± 119 | 4603 ± 1493 | < 0·0001 |
| Tumour necrosis factor-α | 12296 ± 13574 | 34287 ± 40166 | 0·26 |
| Interleukin-27 | 6000 ± 2761 | 7306 ± 3012 | 0·54 |
Monocyte-derived dendritic cells were generated from six donors and their cytokine secretion in response to lipopolysaccharide was determined in the absence or presence of Wortmannin. Shown is the cytokine secretion at 24 hr as determined by ELISA ± SD