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. 2011 Sep;134(1):93–106. doi: 10.1111/j.1365-2567.2011.03471.x

Figure 4.

Figure 4

G-1-induced interleukin-10 (IL-10) production is extracellular signal regulated kinase (ERK) dependent. CD4+ CD44lo CD62Lhi naive CD4+ T cells were collected by FACS and cultured for 4 days ex vivo with anti-CD3/28 + IL-6 + transforming growth factor-β (TGF-β) in the presence of 100 nm G-1 (black bars) or DMSO (white bars). Cultures were supplemented with inhibitors of the ERK (PD98059), Jun N-terminal kinase (JNK II inhibitor), or p38 (SB203580) signalling cascades. Following culture, cells were collected and stained for intracellular IL-10 and IL-17A, and analysed by flow cytometry. (a) Graphs represent summary of data from three independent experiments. (b) Representative plots from one of four independent experiments with PD98059. (c) Summary of data from four PD98059 experiments analysing induction within populations expressing combinations of IL-10 and IL-17A. P-values determined by Student's t-test; *P < 0·05; **P < 0·005; ***P < 0·0005. Error bars = SEM; NS, not significant.