Table 2.

The EFFECT of TUNICAMYCIN on 3H-GLUCOSAMINE INCORPORATION in cumulus, ooplasm and PVS, as observed on semi-thin sections

			Labeling (2)
Oocyte stage at onset of experiment	Duration of culture (hr)	Culture medium (1)	Cumulus cells	ooplasm	PVS
GV (0 hr post hCG)	1		++	+	+
	1+3CH		++	+	+
	20		++++	++	+++
	20+24CH		+++	+	+++
	40			++	+++
	40 (H)			+	+
	40	T		++	++++
	40 (H)	T		+	+
LD (20 hrs post hCG)	1		++	+	+
	1+3CH		++	+	++
	24			++	+++
	24 (H)			+	+
	24	T		++	++++
	24 (H)	T		+	+

(1) The modified TC 199 was supplemented with ³H-glucosamine in the presence (T) or absence of Tunicamycin (2) Same scale of labeling as in Table 1 CH = chase (H) = hyaluronidase treatment at the end of culture