Figure 7.

Molecular identity of voltage-gated Na⁺ channels in human pulmonary artery smooth muscle cells (PASMC). (a) Structural arrangement of Na⁺ channel α-, β₁- and β₂-subunits. (b) The mRNA expression of cloned Na⁺ channels in human brain (Br) and PASMC (PA). Polymerase chain reaction (PCR)-amplifi ed products displayed for the transcripts of SCN2A, SCN4A, SCN5A, SCN6A and β-actin. “-RT”, PCR performed with no reverse transcriptase (RT). “M”, 100 bp DNA ladder. (c) A phylogenetic tree showing the inferred evolutionary relationships among different Na⁺ channel genes