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. 2011 Jul 29;30(18):3830–3841. doi: 10.1038/emboj.2011.249

Figure 3.

Figure 3

MiR-103 does not affect Cav1.2, Cav2.2 and Cav3.2 trafficking. We assessed Cav1.2, Cav2.2 and Cav3.2 trafficking by measuring the ratio between membrane and cytoplasm immunostaining with and without miR-103 application. Membrane is identified with a lipophilic dye, DiD (blue in A, C, E). (A, B) miR-103 over-expressing neurons (red, A, star) showed an overall decrease in Cav1.2 labelling (green, B, arrow and arrowhead) but trafficking was not affected. In contrast, miR-103 over-expression (red, star in C, E) did not alter Cav2.2 nor Cav3.2 labelling (green; D, F). (G) Statistical analysis showed no difference in Cav1.2, Cav2.2 and Cav3.2 trafficking, data are expressed as mean values±s.e.m. n=10 for each condition, bar=20 μm.