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. 2011 Jul 29;30(18):3701–3713. doi: 10.1038/emboj.2011.254

Figure 3.

Figure 3

Deglycosylation of Rh1 is essential for transport, but not required for membrane localization. (A) Time course of Rh1 processing in wild-type flies. Pupal heads were collected at the indicated time. The amount loaded is labelled on the top. (B) The Rh1 deglycosylation process is disrupted in dmppe mutant. Pupae were collected at different pupal development time points. (C) Rh1 distribution in the developing photoreceptors of wild-type flies. Sections were prepared as described in Materials and methods. Cross-sections were stained with anti-Rh1 antibody (green) and rhodamine-phalloidin (red). % pd: % of pupal development. Scale bar, 5 μm. (D) Rh1 distribution in the developing photoreceptors of the dmppe mutant. Scale bar, 5 μm. (E) Quantification of the percentage of Rh1 in rhabdomere during pupal development. Quantification was performed as described in Materials and methods. (F) Most Rh1 localized normally in matured mutant photoreceptors. Flies were raised in the dark and examined at 2 days after eclosion. Collection and fixation were performed under dim red light. Scale bar, 5 μm. (G) Constant Rh1 levels in adult mutants. Flies were raised in the dark and examined at the indicated time after eclosion. The scaffold protein INAD was probed in parallel as a loading control.