Figure 3.

Mapping of specific residues on VEEV E1 and E2. (A) Model of an E1-E2 heterodimer. Two N-linked glycosylation sites (E1-N134 and E2-N318) are labelled in green and red, respectively. The major determinant of virulence attenuation (residue E2-T120) is labelled in dark blue. Two sets of residues (E2-193/213 and E2-218) whose mutations strongly affects the equine and mosquito host range are labelled in grey and black, respectively. The previously identified VEEV epitopes for murine monoclonal antibodies mMAbs (residues 182–207) and human hMAbs (residues 115–119) are coloured in orange and yellow, respectively. (B) Model of an E2 homotrimer of VEEV in one asymmetric unit. The residue labelling is the same as (A).