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. 2011 Aug 9;30(18):3854–3863. doi: 10.1038/emboj.2011.261

Figure 4.

Figure 4

The presence of E3 protein in mature VEEV. (A) The densities for E3 (orange) in one asymmetric unit of the original 3D reconstruction. Note the E3 densities are displayed at slightly lower isosurface threshold than E1 (magenta) and E2 (cyan) densities. Scale bar: 2 nm. (B, C) Side and top views of the density for E3 in the asymmetric unit averaged map. The crystal structures of CHIKV pE2 (orange) and E1 (blue) (PDB code: 3N40) are fitted separately as a rigid body into the averaged density. Our models of VEEV E1, E2 and E3 are shown in magenta, cyan and green, respectively. The blue arrows point to the two rod-like features in the density. (D) SDS–PAGE result of VEEV TC-83 samples we used for imaging. The leftmost and rightmost lanes are molecular size markers. Lanes 1–4 are the four batches of VEEV TC-83 samples used for cryo-EM imaging.