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. 2011 Aug 5;30(18):3823–3829. doi: 10.1038/emboj.2011.263

Figure 2.

Figure 2

A cluster of FBEs in the cki-2 3′UTR mediates repression in germline stem cells. (A) The cki-2 3′UTR mediates repression in stem cells. A GFP–H2B reporter (depicted schematically) is repressed in stem cells when coupled to the cki-2 3′UTR, but expressed ubiquitously when coupled to the non-regulated tbb-2 3′UTR. (B) A region required for repression harbours potential PUF binding elements. Graphical representation of the cki-2 3′UTR (top). Shown are relative locations of MRE1, MRE2 and FBE1–4 elements, the extent of deletions tested in reporter constructs, and the effect of the deletion on distal reporter expression. Deleting FBE1–4 is sufficient for distal derepression of the reporter (bottom). Partial (B) or complete (A) gonads (outlined) from live animals are shown. Scale bars: 50 μm.