Table 1. Quantification of FBF-2 association with FBEa and FBE1–FBE4.

Oligo name	Kd,app(GS)	Kd,app(FP)
gld-1 FBEa	32±1 nM	11±2 nM
cki-2 FBE1	26±12 nM	5±1 nM
cki-2 FBE1 mut	> 500 nM	> 500 nM
cki-2 FBE2	> 500 nM	> 500 nM
cki-2 FBE2 mut	> 500 nM	> 500 nM
cki-2 FBE3	240±8 nM	114±2 nM
cki-2 FBE3 mut	NB	>500 nM
cki-2 FBE4	300±40 nM	95±3 nM
cki-2 FBE4 mut	NB	>500 nM
Affinities of FBF-2 to indicated RNA elements as determined by F-EMSA and FP assays. NB, no binding. Kd,app, apparent equilibrium dissociation constant. For oligo sequences, see Supplementary Table S1.