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Specificity of GrB and IFN-γ secretion by αFMP-CTL in the ELISPOT assays. Human αFMP-CTL (7 day culture, 5 × 103 cells per well) were run alone (A) or against various target cells (5 × 104 cells per well): C1R.A2 (B), C1R.A2 pulsed with 5 μg/ml FMP (C) or C1R.A2 pulsed with 3 μM MART-1 (D). Effector and target cells were incubated for 4 h at 37°C assays. Data is presented as spots per well ± SD and is representative of 3 experiments.
