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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Genes Immun. 2011 Jun 9;12(7):575–581. doi: 10.1038/gene.2011.12

Figure 4. Dynamics of STAT3, STAT1 and JAK1 phosphorylation in HeLa cells expressing wildtype or mutant IL10R1.

Figure 4

HeLa cells transfected with IL10R1-WT,-SNP3, -SNP4 or –SNP3+4 were cultured in the absence (−) or presence of IL-10 (IL-10 cont.), or treated with an IL-10 pulse for 30 min (IL-10 pulse). After 0.5, 3, 6, 9, 12 and 24h, cells were lysed and phosphorylation of STAT3 at Tyr705 (a) and STAT1 at Tyr701 (b) was detected by western blot. (c) JAK1 phosphorylation was detected after 6h (6h IL-10 or 30min IL-10 pulse).

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