Table I.
Mass Spectrometric Transitions at Specific Collision Energies (CE), Retention Times (Rt), and Imprecision (%CV) after 50 Injections for Morphine, Codeine, 6-Acetylmorphine (6AM), Benzoylecgonine (BE), Cocaine, 2-Ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), Methadone, and Deuterated IStd
| Segment | Compound | Quantifier Transition (CE) | Qualifier Transitions (CE) | Rt (min) | %CV Rt (n = 50) |
|---|---|---|---|---|---|
| I | Morphine | 286.2 → 201.1 (37) | 286.2 → 229.0 (37) | 4.50 | 0.74 |
| 286.2 → 268.1 (37) | |||||
| Morphine-d6 | 292.3 → 201.1 (37) | – | 4.48 | 0.71 | |
| II | Codeine | 300.2 → 215.1 (37) | 300.2 → 243.0 (37) | 5.37 | 0.3 |
| 300.2 → 282.2 (37) | |||||
| Codeine-d6 | 306.2 → 218.1 (37) | – | 5.35 | 0.31 | |
| 6AM | 328.2 → 211.1 (38) | 328.2 → 268.1 (38) | 5.66 | 0.25 | |
| 328.2 → 193.1 (38) | |||||
| 6AM-d6 | 334.1 → 211.1 (38) | – | 5.64 | 0.25 | |
| BE | 290.1 → 168.1 (29) | 290.1 → 168.1 → 150.0 (32) | 6.10 | 0.20 | |
| 290.1 → 168.1 → 82.1 (32) | |||||
| BE-d8 | 298.2 → 171.1 (29) | – | 6.07 | 0.20 | |
| III | Cocaine | 304.1 → 182.1 (30) | 304.1 → 182.1 → 150.1 (32) | 6.92 | 0.22 |
| 304.1 → 182.1 → 82.1 (32) | |||||
| Cocaine-d3 | 307.1 → 185.1 (30) | – | 6.91 | 0.24 | |
| IV | EDDP | 278.2 → 249.1 (40) | 278.2 → 249.1 → 234.2 (34) | 8.61 | 0.50 |
| EDDP-d3 | 281.3 → 249.1 (40) | – | 8.60 | 0.49 | |
| Methadone | 310.1 → 265.0 (27) | 310.1 → 265.0 → 247.1 (31) | 8.72 | 0.62 | |
| 310.1 → 265.0 → 219.1 (31) | 8.69 | 0.65 | |||
| Methadone-d9 | 319.2 → 268.1 (27) | – |