Table III.
Imprecision and Analytical Recovery for Morphine, Codeine, 6-Acetylmorphine (6AM), Benzoylecgonine (BE), Cocaine, 2-Ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and Methadone
| Imprecision* |
Analytical Recovery† |
|||||
|---|---|---|---|---|---|---|
| Compound | QC | Intra-assay (n = 5) | Interassay (n = 20) | Total | Intra-assay (n = 5) | Interassay (n = 20) |
| Morphine | Low | 5.9 | 0.0 | 5.9 | 101.8 | 100.4 |
| Medium | 4.9 | 5.0 | 7.01 | 92.9 | 100.7 | |
| High | 7.9 | 4.1 | 8.9 | 92.8 | 97.1 | |
| Codeine | Low | 8.7 | 4.1 | 9.6 | 111.7 | 104.2 |
| Medium | 7.0 | 3.6 | 7.9 | 113.3 | 106.6 | |
| High | 5.7 | 6.8 | 8.8 | 112.1 | 103.5 | |
| 6-AM | Low | 7.8 | 7.2 | 10.6 | 89.9 | 98.1 |
| Medium | 4.5 | 0.0 | 4.5 | 111.5 | 110.2 | |
| High | 8.2 | 4.0 | 9.1 | 93.1 | 100.3 | |
| BE | Low | 8.6 | 5.1 | 10.0 | 92.4 | 97.9 |
| Medium | 6.2 | 6.7 | 9.1 | 110.7 | 101.2 | |
| High | 8.1 | 4.7 | 9.4 | 92.3 | 97.6 | |
| Cocaine | Low | 5.6 | 3.0 | 6.3 | 110.1 | 105.0 |
| Medium | 2.7 | 0.0 | 2.7 | 111.6 | 107.1 | |
| High | 4.7 | 4.1 | 6.2 | 109.7 | 102.7 | |
| EDDP | Low | 5.3 | 9.1 | 10.5 | 87.5 | 94.5 |
| Medium | 4.2 | 5.3 | 6.8 | 87.7 | 92.6 | |
| High | 5.0 | 9.8 | 11.1 | 84.4 | 92.2 | |
| Methadone | Low | 5.7 | 1.8 | 5.9 | 95.7 | 99.1 |
| Medium | 6.5 | 5.8 | 8.7 | 92.7 | 96.5 | |
| High | 4.9 | 6.3 | 8.0 | 112.1 | 100.2 | |
*
Imprecision is expressed as %CV.
†
Analytical recovery is expressed as % of target concentration.