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Effect of Mg2+ and ATP concentrations on the rate of PFK-catalyzed reaction. For (○), Mg2+ ion concentration was equal to and varied together with that of ATP. For (•), Mg2+ ion concentration was equal to 3.33 mM. F-6-P concentration in each case was 3.33 mM. Enzyme concentration was 10 μg/mL. Other conditions were same as in standard enzyme assay.
