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Effect of PEP on the rate of PFK-catalyzed reaction. PEP concentrations were nil (□), 1.0 (○), 2.0 (•), and 5.0 (Δ) mM. Mg2+ and ATP concentrations were 3.3 and 0.1 mM, respectively. Enzyme concentration was 10 μg/mL. Other conditions were same as in standard enzyme assay.
