Figure 2.

MAB17781 inhibits cytokinesis of adherent HeLa cells. The β1-blocking antibody MAB17781 (MAB) and IgG control isotype antibody were added to a sub-confluent culture of HeLa cells 5 hr post-seeding. (A) Flow cytometry histograms of IgG (control) or MAB-treated cells, 48 hr post-treatment, indicate a rise in the G₂ (binucleated cells) subpopulation in the presence of MAB17781. (B) Tabulated results from experiments in (A) done in triplicate and represented as the mean ± SEM (p < 0.001, Student's t-test) have been presented, confirming cytokinesis failure as evidenced by an increase in the G₂ subpopulation induced by MAB17781. (C) Western blot analysis of IgG or MAB-treated cells, 48 hr post-treatment, indicate that MAB treatment does not affect steady state levels of CrkII, CrkI or paxillin. GAPDH was used as the loading control.