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. 2011 Aug 22;121(Pt 12):565–572. doi: 10.1042/CS20110236

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© 2011 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Membrane fractions were prepared from washed RBCs (A) or liver tissue (B). S1P phosphatase (P'tase) and S1P lyase activities were quantified by measuring the rates of formation of sphingosine and hexadecenal (detected as its semicarbazone) as described in the Materials and methods section.