Figure 3. Lucanthone/Hycanthone promote APE1 cleavage at lower concentration than CRT.

Western blot of APE1 over expresser clone 5 pretreated with increasing concentration of lucanthone (2.5–100 µM) and CRT0044876 (2.5–200 µM) in presence of protease inhibitor cocktail for 2 h at 37°C (10 µg of total cell protein loaded per lane). The corresponding decrease in 35.5 kDa APE1 protein band and increase in 25-kDa-degradation product is indicated by the arrows.