Figure 14. Lucanthone causes cleavage of APE1 with intact hydrophobic site.

(A). Western blot of recombinant and mutant APE1 proteins (upper panel) treated with 100 µM lucanthone (lower panel) at 37°C for 2 h. (B). Endonuclease activity inhibition of wild type and F266A mutant of APE1 in presence of 100 µM of lucanthone for 2 h at 37°C.