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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cell Mol Biol (Noisy-le-grand). 2011 May 24;57(Suppl):OL1480–OL1486.

Figure 1. Expression of whole-cell amiloride-sensitive currents in primary cultured osteoblasts.

Figure 1

Representative recordings (A) and mean values (B–C) of whole-cell currents in osteoblasts and representative current-voltage (D) after using 20 nM 17 β-estradiol(E2). A: cell was held at a potential of −40 mV. Currents were elicited by applying voltage steps between −100 and +100 mV in 10-mV steps lasting 500 ms every 10 s. Pipette was filled with standard internal solution, and cell was perfused with standard external solution (see MATERIALS AND METHODS for details). Currents were recorded after perfusion of cell with the bath solution containing 1 mM amiloride. Amiloride-sensitive currents (AS) were obtained by subtracting amiloride-resistant currents (AR) from total current (Total). B. Mean values of whole-cell currents in osteoblast cells recorded in −100 mV. C: current-voltage (I-V) relationships of Total, AR, and AS currents. Values are means ± SE; n =4 cells. D. Cell was held at a potential of −40 mV. Currents were elicited by applying voltage steps between −100 and +100 mV in 10-mV steps lasting 500 ms every 10 s. Currents were recorded after perfusion of cell with the bath solution (control), the bath solution containing 20 nM 17 β-estradiol (20 nM E2), the bath solution containing 1 mM amiloride (plus 1mM Amil).