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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cell Mol Biol (Noisy-le-grand). 2011 May 24;57(Suppl):OL1480–OL1486.

Figure 2. Analysis of ENaC mRNA expression in primary mouse osteoblasts.

Figure 2

Total RNAs were isolated according to manufacture instruction. Messenger RNA levels for ENaC α, γ were determined by RT-PCR. The levels of mRNA were normalized to that of 18S ribosome RNA. Three independent experiments were carried out (* represents P<0.05, ** represents P<0.01). BC, MC and E2 individually represent blank control group match control group and the estrogen (10−5 mol/L) group.