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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cell Mol Biol (Noisy-le-grand). 2011 May 24;57(Suppl):OL1480–OL1486.

Figure 3. Analysis of ENaC protein expression in primary mice osteoblasts.

Figure 3

Proteins in the same condition as RT-PCR studies were extracted as methods. ENaC α, γ were determined by western-blot using immunopurified anti-goat ENaC antibodies. The levels of proteins were normalized to that of β-actin as an internal standard. No background staining was seen when omitting the primary antibody (data not shown) (* represents P<0.05, ** represents P<0.01).