Figure 1. Fluorescence spectra of purified Peak 1 CFP-II^Glc plus Peak 1 YFP-II^Glc mixture, purified Peak 1 CFP-II^Glc and purified Peak 1 YFP-II^Glc.

(A) The two fluorescent proteins were not detergent-treated, but were mixed before purification at a 1:6 ratio (Both; dark line). Alternatively, the spectrum of pure Peak 1 CFP-II^Glc (CFP, light line) or pure Peak 1 YFP-II^Glc (YFP, dotted line) alone was measured. (B) The spectrum of the YFP-II^Glc protein was subtracted from the spectrum of the Peak 1 CFP-II^Glc - Peak 1 YFP-II^Glc mixture (Both corrected, dark line). The purified CFP-II^Glc spectrum is also shown (CFP, light line). (No FRET was observed).