| 1 |
Fluorescence energy transfer (FRET) |
Protein:protein interactions. Peak 2 can associate to peak 1 without detergent. Peak 1 cannot dissociate to peak 2 without detergent. |
| 2 |
Formaldehyde cross linking |
Protein:protein interactions. In vivo: peak 1 becomes insoluble with formaldehyde but peak 2 does not. In vitro: peak 1 is inactivated; peak 2 is not. |
| 3 |
Enzyme activity |
Dependency of substrate inhibition on monomeric versus dimeric form: identification of dimeric micellar IIGlc. |
| 4 |
Cryo-electron microscopy (C-EM) and dynamic light scattering (DLS) |
Purified French pressed membrane vesicles are heterogeneous; sizes: pellet> peak 1> peak 2 |
| 5 |
Nuclear magnetic resonance (NMR) |
Pellet is only in bilayer form. Peak 1, leading edge: only in bilayer form; peak 1 central part: mixture of bilayer form and dimeric micelles; peak 1 trailing edge: dimeric micelles. Peak 2 monomeric micelles. |
| 6 |
Lipid measurements |
Pellet: PE/PG = 1.8Peak 1: PE/PG = 1.0Peak 2: PE/PG = 1.0 |
| 7 |
Lipid/protein measurements |
Pellet: lipid/protein (ng/µg) = 35Peak 1: lipid/protein (ng/µg) = 12Peak 2: lipid/protein (ng/µg) = 5 |
