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. 2011 Sep 15;6(9):e24263. doi: 10.1371/journal.pone.0024263

Figure 4. Effect of neurogenesis ablation on spatial memory and depression-like behavior.

Figure 4

(A) A schematic diagram of intraventrucular infusion and treatment procedure; CORT treatment was started after two days of recovery from the surgical procedure. The behavioral test was then performed 24 hr after the treatment. MWM: Morris water maze (B–F) Representative images of BrdU staining. (G) Ara-c infusion significantly blocked hippocampal cell proliferation. (H) Spatial learning for different treatment groups. (I) Ara-c infusion did not affect spatial learning in vehicle-treated runners. (J) Blocking hippocampal neurogenesis in 40 mg/kg CORT-treated runners significantly impaired spatial learning compared to saline infused counterparts. Main effect of Ara-c infusion, * P<0.05 (K and L) Data were analyzed with the modified method. Memory consolidation was not altered by Ara-c infusion in either vehicle- or 40 mg/kg CORT-treated runners as indicated by analysis of conventional and modified method respectively in probe trial test. (M) Ara-c infusion significantly increased immobility time in 40 mg/kg CORT-treated runners indicating increased depression-like behavior. Main effect of Ara-c: **P<0.005. SA: Saline infusion; AC: Ara-c infusion. Scale bar  = 100 µm. N = 8–10/group.