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. 2011 Sep 15;6(9):e24423. doi: 10.1371/journal.pone.0024423

Figure 1. Hippocampal GluN1 -/- neurons develop normal synapse density in an equal co-culture with wild type neurons.

Figure 1

(A) Hippocampal neurons from littermate wild type or GluN1 -/- mice were labeled by nucleofection and grown in co-cultures as indicated. (B) There was no significant difference in cell survival at 14 DIV according to genotype or culture composition. Values indicate number and percent of cells per microscope field labeled with YFP or CFP representing the indicated genotype; the 50% WT and 50% KO data come from the same coverslips of YFP WT mixed with CFP KO and of CFP WT mixed with YFP KO; ANOVA p>0.05; n = 60 from two independent experiments. (C) At 14 DIV, neurons were fixed and immunolabeled for synaptic markers. Neurons of defined genotype were identified by the YFP or CFP dendrite fill. Synapse density appeared similar regardless of genotype and culture composition. (D) There was no significant difference according to genotype or culture composition in synapsin puncta marking total synapse density or in colocalized PSD-95 and VGlut1 puncta marking excitatory synapse density; the 50% WT and 50% KO data come from the same coverslips of YFP WT mixed with CFP KO and of CFP WT mixed with YFP KO; ANOVA p>0.1; n≥50 from 3 independent experiments. All data are presented as mean ± SEM.