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. 2011 Sep 15;6(9):e24423. doi: 10.1371/journal.pone.0024423

Figure 4. Synaptic competition in wild type and GluN1 -/- neuron co-culture is dependent on NMDA receptor activity.

Figure 4

Hippocampal neurons from littermate wild type or GluN1 -/- mice were labeled by nucleofection and grown in coculture as in Figure 2, except that the NMDA receptor antagonist MK-801 was added chronically from DIV 0. At 14 DIV, neurons were fixed and analysed as in Figure 3. There was no significant difference among coculture conditions in density of PSD-95 puncta, VGlut1 puncta, and colocalized PSD-95 and Vglut1 puncta marking excitatory synapses; ANOVA p>0.1; n = 30 from 2 independent experiments (except n = 15 for 100% WT). Data are presented as mean ± SEM.