Figure 3. Recombinant MBP-hNaa40p acetylates H2A and H4 N-termini in vitro.

MBP-hNaa40p was incubated with [1-¹⁴C]-Ac-CoA and selected peptides of 24–28 amino acids, representative of different protein N-termini. The first four N-terminal residues are shown for each substrate. The Nt-Ac-H4 substrate is a histone H4 peptide where the N-terminus is blocked by an acetyl group. Incorporated acetyl groups were measured as DPM. Error bars indicate standard deviation for at least three independent acetylation reactions.