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. 2011 Sep 15;6(9):e24713. doi: 10.1371/journal.pone.0024713

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Hole et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A431 cells were harvested and fractionated. Total cell lysate (T), cytosolic fraction (C), and nuclear fraction (N) were analyzed by SDS-PAGE and western blotting. Beta-tubulin was used as a cytosolic marker, while histone H4 was used as a nuclear marker. hNaa40p was detected in all fractions using an anti-hNaa40p antibody. Results shown are representative of three independent experiments.