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. 2011 Sep 15;6(9):e24856. doi: 10.1371/journal.pone.0024856

Figure 6. IL-17 is expressed in NSCLC and induces CCL20 production by NSCLC-derived cell lines.

Figure 6

PCR signal for IL-17A, IL-17A receptor and b-actin in NSCLC tissue samples, in tumor adjacent lung tissue and in L3, L4 and A549 cell lines. Tumor adjacent lung tissue (A). NSCLC tumor sample (B). L3, L4 and A549 cell lines (C). Infiltrating immune cells were isolated from tumor tissue (TD-IC) and tumor adjacent lung tissue (LD-IC). Immune cells were incubated with or without anti CD3 antibody and production of IL-17 assessed by ELISA - two cases (D, E). Semi-quantitative PCR analysis for CCL20 in L3, L4 cells 549 cells in response to IL-17 and IL-22 stimulation (F, G, H). ELISA assays for CCL20 in the supernatant of L3, L4 cells 549 cells following stimulation with increasing concentrations of IL-17 (I, J, K).