Figure 3. The effect of DIDS on GABAAR currents.
A, An example recording and mean data (n = 4) showing that application of DIDS (500 µM) in the presence of TPMPA (200 µM) but not bicuculline has no effect on the holding current but inhibits spontaneous GABAAR-mediated IPSCs (sIPSCs). B, Example current traces from recordings with and without DIDS (500 µM) included in the intracellular solution, with average sIPSCs from a different recording with intracellular DIDS (500 µM), and mean sIPSC amplitude and frequency data in control recordings (n = 5) and recordings with intracellular DIDS (0.5–1 mM; n = 5). Control(1) and DIDS(1) were measured during the 2nd minute after gaining whole-cell access, DIDS(2) was measured during the 6th minute.