Figure 5. Cyclothiazide-sensitivity of GABA_CR-currents.

A, Example experiment showing the effect of cyclothiazide (CTZ; 300 µM) on the holding current, with subsequent application of NO-711 (3 µM) and picrotoxin (250 µM), with mean data for these experiments (n = 10). B, Example synaptic feedback currents evoked by brief BCT depolarization (to −10 mV for 5 ms) during wash-on of cyclothiazide, and mean data showing the biphasic effect of cyclothiazide on the charge of feedback responses (n = 8). CTZ(1) was measured 3–4 mins after application, CTZ(2) was measured 6–8 mins after application. C, Example GABA-evoked responses (100 µM, 50–100 ms) showing the effects of cyclothiazide and NO-711 on response amplitude (top) and kinetics (bottom, peak-scaled responses from a different experiment), with mean data (n = 7) for the charge and decay time-constant of GABA-evoked responses under these conditions.