Figure 4. Adoptively transferred DCs migrate from the periphery into the inflamed brain.

A) Transferred DCs (CD45.1) recovered from the brain, spleen, lymph nodes and peripheral blood of recipient animals 18–24 hours later. The expression of CD11c, CD11b, MHCII, and Ly6C on the CD45.1⁺ fraction (transferred population) is shown in the panels on the right. B) Whole organ imaging using a Licor Odyssey imager of the brain, spleen and cervical lymph nodes from naïve or infected mice that received DCs loaded with near infrared dye labeled polymersomes. C) Snapshot showing TRITC labeled DCs and OT1^GFP T cells in a brain slice from a Pru^OVA infected mouse. D) Snapshot showing transferred DCs (red), OT1^GFP T and endogenous CD11c^YFP cells in the infected brain. E) The average migration velocity of transferred DCs (red triangles) and endogenous CD11c^YFP cells (yellow triangles).