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. 2011 Sep 15;7(9):e1002253. doi: 10.1371/journal.ppat.1002253

Figure 4. Microbiocidal activity and limited bacteria-cell contact does not provide for decreased phagocytic clearance of non-swimming bacteria.

Figure 4

(A) Adherent murine peritoneal macrophages were co-incubated with PA14 WT or motABmotCD and cellular associated bacteria and non-associated bacteria were quantitatively assessed at the indicated time points. (B) BMDCs were co-incubated with WT or motABmotCD in the presence or absence of 0.01% Tween80, 0.01% beta-octyl glucoside, or 2% Survanta, and assayed by gentamicin protection assay for relative bacterial phagocytosis. (C, inset) GFP-expressing PA14 WT or motABmotCD were centrifuged onto BMDCs and immediately fixed and analyzed by FACs for cellular association. Phagocytic cells in the absence of bacteria are shown as background. (C) P. aeruginosa PA14 WT, flgK, or motABmotCD, or (D) V. cholerae O395 WT, flaA, or motX were centrifuged onto BMDCs or peritoneal macrophages, respectively, and assayed by gentamicin protection assay. N≥5, *p<0.05 as compared to WT.